HEPES (4- (2-hydroxyethyl) -1-piperazineethanesulfonic) a zwitterionic amoebic actinic abettor buffer; a absorber of twenty well.
HEPES is frequently acclimated in corpuscle culture, abnormally because it is bigger to a physiological pH, admitting changes in the assimilation of carbon dioxide (produced by cellular respiration) authority compared to bicarbonate buffers, which are aswell frequently acclimated in corpuscle culture.
The break of baptize increases with temperature drop, but the break connected (pK) of abounding added absorber with the temperature does not change much. HEPES is like baptize that its break decreases as the temperature decreases.
more about: HEPES
from: Pharmaceutical Ingredients
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